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Following morphological measurements and chromosomal counts, carried out on native populations in Austria, Bulgaria, Croatia, the Czech Republic, Denmark, Estonia, Germany, Hungary, Ireland, Italy, Lithuania, Luxembourg, the Netherlands, Norway, Portugal, Rumania, Slovakia, Slovenia, Spain, Sweden and Switzerland, which confirmed six taxa within the Eleocharis palustris agg. in Europe (Bureš 1998, 1999, 2002), we have focused on the study of relative DNA content variability of these taxa using DAPI flow cytometry. Both subspecies of E. mamillata, which have the same dominant chromosome number 2n=16, have very similar DNA contents. On the other hand, closely related E. palustris subsp. palustris in which 2n=16 also dominates, has a smaller DNA content than either subspecies of E. mamillata. It is also unusual that E. palustris subsp. vulgaris with a dominant 2n=38, 39 has a larger DNA content than E. uniglumis subsp. uniglumis, which has a dominant 2n=46. E. uniglumis subsp. sterneri, described by Strandhede in Bot. Not. 114: 433 (1961) as endemic to the islands of Öland and Gotland (Baltic Sea) and confirmed later by Bureš (1998, 1999) from Slovakia, Austria, Hungary and Croatia, has large variability of chromosome numbers (Strandhede 1965, Bureš 1998). Likewise DNA content variability of E. uniglumis subsp. sterneri is very large in relation to the other five taxa. Our study was supported by the Ministry of Education of the Czech Republic (research project MSM 143100010 Spatial and Temporal Biodiversity Dynamics in Ecosystems of Central Europe).
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