Application of micellar electrokinetic capillary chromatography for the analysis of schizandra extract
| Autoři | |
|---|---|
| Rok publikování | 2001 |
| Druh | Článek ve sborníku |
| Konference | Book of abstract ICES 2001 An Electrophoretic Odessey |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Biochemie |
| Klíčová slova | MEKC Schizandra Lignans |
| Popis | Schisandra chinensis is a famous medicinal plant native to East Asia. The biologically active compounds are the lignans with an uncommon structure derived from dibenzo[a,c]cyclooctadiene. The lignans prevent liver injuries, stimulate liver regeneration and inhibit hepatocarcinogenesis and lipid peroxidation. Moreover several reports indicate, that the lignans have effects on human intellectual activity and physical performance. Recently, dibenzo[a,c]cyclooctadiene lignans were identified as potent anti-HIV agent. MEKC has been used for the analysis of the Schisandra extract. An extract was dried and disolved in 10 mM disodium tetraborate buffer pH 9,3 containing of 20 mM SDS and 35 % (v/v) acetonitrile. A buffer consisting of 40 mM SDS and 35 % (v/v) acetonitrile in 10 mM disodium tetraborate pH 9,3, an applied voltage of 28 kV (positive polarity) and a temperature of capillary 25 oC gave the best separation of the lignans. |
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