Strategies for labelling of exogenous and endogenous extracellular vesicles and their application for in vitro and in vivo functional studies

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Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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BOUDNÁ Marie DELGADO CAMPOS Andres VYCHYTILOVÁ Petra MACHÁČKOVÁ Táňa SLABÝ Ondřej SOUČKOVÁ Kamila

Rok publikování 2024
Druh Článek v odborném periodiku
Časopis / Zdroj Cell Communication and Signaling
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-01548-3
Doi http://dx.doi.org/10.1186/s12964-024-01548-3
Klíčová slova Extracellular vesicles; Exosomes; EV tracking; Fluorescent labelling; Bioluminescent labelling; Radiolabelling; Magnetic resonance; In vivo imaging; Cre-loxP; CRISPR-Cas
Přiložené soubory
Popis This review presents a comprehensive overview of labelling strategies for endogenous and exogenous extracellular vesicles, that can be utilised both in vitro and in vivo. It covers a broad spectrum of approaches, including fluorescent and bioluminescent labelling, and provides an analysis of their applications, strengths, and limitations. Furthermore, this article presents techniques that use radioactive tracers and contrast agents with the ability to track EVs both spatially and temporally. Emphasis is also placed on endogenous labelling mechanisms, represented by Cre-lox and CRISPR-Cas systems, which are powerful and flexible tools for real-time EV monitoring or tracking their fate in target cells. By summarizing the latest developments across these diverse labelling techniques, this review provides researchers with a reference to select the most appropriate labelling method for their EV based research.
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