A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines

• Autoři: BINÓ Lucia; MIKULENKOVÁ Erika; ŠTĚPÁNEK Luděk; BERNATÍK Ondřej; VYSLOUŽIL David; PEJŠKOVÁ Petra; GORILÁK Peter; HURANOVÁ Martina; VARGA Vadimír; ČAJÁNEK Lukáš
• Rok publikování: 2022
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: STAR Protocols
• Fakulta / Pracoviště MU: Lékařská fakulta
• www: https://www.sciencedirect.com/science/article/pii/S266616672200079X
• Doi: http://dx.doi.org/10.1016/j.xpro.2022.101199
• Klíčová slova: Cell Biology; Cell culture; Microscopy; Molecular Biology
• Popis: Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).

Související projekty

• Molekulární aspekty ciliogeneze: zaměření na Tau Tubulin Kinázu 2
• National research infrastructure for biological and medical imaging
• Souhra mezi regulacemi buněčného cyklu a ciliogeneze
• Ciliární Tau tubulin kináza 2 ve zdraví a nemoci: struktura a funkce