The polyvalent staphylococcal phage 812: its host-range mutants and related phages

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Publikace nespadá pod Fakultu sportovních studií, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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PANTŮČEK Roman ROSYPALOVÁ Alena DOŠKAŘ Jiří KAILEROVÁ Jana RŮŽIČKOVÁ Vladislava BORECKÁ Pavla SNOPKOVÁ Šárka HORVÁTH Radek GÖTZ Friedrich ROSYPAL Stanislav

Rok publikování 1998
Druh Článek v odborném periodiku
Časopis / Zdroj Virology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www [Abstract]
Obor Genetika a molekulární biologie
Popis Ninety-five percent of 782 culture collection strains, as well as hospital strains of Staphylococcus aureus subsp. aureus of different provenance and 43% of 89 culture collection strains of different coagulase-negative species of the genus Staphylococcus,were found to be sensitive to the polyvalent phage 812 or to at least one of its host-range mutants or to the polyvalent phages SK311, 131,and U16.Thus sensitivity to the polyvalent staphylococcal phages seems to be one of the common features of S. aureus subsp. aureus strains. The adsorption kinetics and one-step growth characteristics of the phages 812 and SK311 were estimated. Restriction genomic maps of the phages 812 (146.5 kb) and SK311 (141.1 kb) were constructed by use of the restriction endonucleases Ava II, Pst I, Kpn I, Sac I, Sma I, and Xho I. The host-range mutations of the phage 812 were localized on this map. Comparison of restriction patterns of the phages 812 and SK311 with those of the polyvalent phages U16 and 131 suggests that all these phages are closely related. Their genomes differ from each other mostly by some deletions, insertions (1-3 kb), or inversions. Evidence was given that the phage 812 together with SK311,131,and U16 belongs in the phage species Twort, the description of which is substantially supplemented with the data on the phage 812 reported in this paper. Copyright 1998 Academic Press.
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