Microcystin-LR, a cyanobacterial toxin affects root development by changing levels of PIN proteins and auxin response in Arabidopsis roots

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
Autoři

FREYTAG C. MATHE C. RIGO G. NODZYNSKI Tomasz KONYA Z. ERDODI F. CSEPLO A. POZER E. SZABADOS L. KELEMEN A. VASAS G. GARDA T.

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Chemosphere
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://dea.lib.unideb.hu/dea/bitstream/handle/2437/305373/FILE_UP_1_Freytagetal_Chemosphere2021.pdf;jsessionid=DB2D8BA7221EC920651D947922629AD1?sequence=1
Doi http://dx.doi.org/10.1016/j.chemosphere.2021.130183
Klíčová slova Microcystin-LR; Arabidopsis; PIN efflux Carrier; Auxin; Protein phosphatase PP2A; Root development
Popis Microcystin-LR (MCY-LR) is a heptapeptide toxin produced mainly by freshwater cyanobacteria. It strongly inhibits protein phosphatases PP2A and PP1. Functioning of the PIN family of auxin efflux carriers is crucial for plant ontogenesis and their functions depend on their reversible phosphorylation. We aimed to reveal the adverse effects of MCY-LR on PIN and auxin distribution in Arabidopsis roots and its consequences for root development. Relatively short-term (24 h) MCY-LR treatments decreased the levels of PIN1, PIN2 and PIN7, but not of PIN3 in tips of primary roots. In contrast, levels of PIN1 and PIN2 increased in emergent lateral roots and their levels depended on the type of PIN in lateral root primordia. DR5:GFP reporter activity showed that the cyanotoxin-induced decrease of auxin levels/responses in tips of main roots in parallel to PIN levels. Those alterations did not affect gravitropic response of roots. However, MCY-LR complemented the altered gravitropic response of crk5-1 mutants, defective in a protein kinase with essential role in the correct membrane localization of PIN2. For MCY-LR treated Col-0 plants, the number of lateral root primordia but not of emergent laterals increased and lateral root primordia showed early development. In conclusion, inhibition of protein phosphatase activities changed PIN and auxin levels, thus altered root development. Previous data on aquatic plants naturally co-occurring with the cyanotoxin showed similar alterations of root development. Thus, our results on the model plant Arabidopsis give a mechanistic explanation of MCY-LR phytotoxicity in aquatic ecosystems. (c) 2021 The Author(s). Published by Elsevier Ltd.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info