The Arabidopsis condensin CAP-D subunits arrange interphase chromatin

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Publikace nespadá pod Fakultu sportovních studií, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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MUNICIO C. ANTOSZ W. GRASSER K.D. KORNOBIS E. VAN BEL M. EGUINOA I. COPPENS F. BRAUTIGAM A. LERMONTOVA Inna BRUCKMANN A. ZELKOWSKA K. HOUBEN A. SCHUBERT V.

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj New Phytologist
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17221
Doi http://dx.doi.org/10.1111/nph.17221
Klíčová slova Arabidopsis thaliana; chromatin organization; chromosomes; condensin; interphase nuclei; SMC proteins
Popis Condensins are best known for their role in shaping chromosomes. Other functions such as organizing interphase chromatin and transcriptional control have been reported in yeasts and animals, but little is known about their function in plants. To elucidate the specific composition of condensin complexes and the expression of CAP-D2 (condensin I) and CAP-D3 (condensin II), we performed biochemical analyses in Arabidopsis. The role of CAP-D3 in interphase chromatin organization and function was evaluated using cytogenetic and transcriptome analysis in cap-d3 T-DNA insertion mutants. CAP-D2 and CAP-D3 are highly expressed in mitotically active tissues. In silico and pull-down experiments indicate that both CAP-D proteins interact with the other condensin I and II subunits. In cap-d3 mutants, an association of heterochromatic sequences occurs, but the nuclear size and the general histone and DNA methylation patterns remain unchanged. Also, CAP-D3 influences the expression of genes affecting the response to water, chemicals, and stress. The expression and composition of the condensin complexes in Arabidopsis are similar to those in other higher eukaryotes. We propose a model for the CAP-D3 function during interphase in which CAP-D3 localizes in euchromatin loops to stiffen them and consequently separates centromeric regions and 45S rDNA repeats.
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