14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?

Logo poskytovatele

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
Autoři

NÁPLAVOVÁ Alexandra KOZELEKOVÁ Aneta HRITZ Jozef

Rok publikování 2021
Druh Článek ve sborníku
Konference Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf
Klíčová slova 14-3-3; 14-3-3zeta; phosphomimicking mutant; monomerization; thermal stability
Popis The 14-3-3 proteins are typically found in dimeric form crucial for their proper function in cells. Yet, a unique role of monomers has been debated in recent studies. Monomerization of 14-3-3? is regulated via phosphorylation of Ser58 located at dimer interface. However, it is difficult to obtain 14-3-3? specifically phosphorylated at Ser58 (pS58) in sufficient purity and amount. For these reasons three distinct phosphomimicking mutants, i. e. 14-3-3? S58E, S58D and S57D S58D were designed and studied in terms of the oligomeric nature and thermodynamical properties. Finally, they were compared to 14-3-3? wild type and monomeric mutant L12E M78K, used to approximate monomeric 14-3-3? pS58, to discuss whether they could be valid alternative for study of 14-3-3? pS58. Although phosphomimicking mutants S58E and S58D and 14-3-3? pS58 share negative charge at position 58, they were observed to exhibit distinct properties than monomeric 14-3-3?. Designed double mutant S57D S58D with additional negative charge in the Ser58 surrounding appeared as more appropriate alternative of pS58 protein.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info