Analysis of Bacteriophage-Host Interaction by Raman Tweezers

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Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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PILAT Zdenek JONAS Alexandr PILATOVA Jana KLEMENTOVA Tereza BERNATOVA Silvie SILER Martin MANKA Tadeas KIZOVSKY Martin RŮŽIČKA Filip PANTŮČEK Roman NEUGEBAUER Ute SAMEK Ota ZEMANEK Pavel

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Analytical chemistry
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://doi.org/10.1021/acs.analchem.0c01963
Doi http://dx.doi.org/10.1021/acs.analchem.0c01963
Klíčová slova Infectious diseases; Viruses; Bioinorganic chemistry; Genetics; Raman spectroscopy
Popis Bacteriophages, or "phages" for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.
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