Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages

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Publikace nespadá pod Fakultu sportovních studií, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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SOCHOR Jiri RYVOLOVA Marketa KRYSTOFOVA Olga SALAS Petr HUBALEK Jaromir ADAM Vojtech TRNKOVÁ Libuše HAVEL Ladislav BEKLOVÁ Miroslava ZEHNALEK Josef PROVAZNIK Ivo KIZEK René

Rok publikování 2010
Druh Článek v odborném periodiku
Časopis / Zdroj Blood Cells, Molecules and Diseases
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://www.mdpi.com/1420-3049/15/12/8618
Doi http://dx.doi.org/10.3390/molecules15128618
Klíčová slova antioxidant activity; spectrometry; trolox; gallic acid
Popis The aim of this study was to describe behaviour, kinetics, time courses and limitations of the six different fully automated spectrometric methods - DPPH, TEAC, FRAP, DMPD, Free Radicals and Blue CrO(5). Absorption curves were measured and absorbance maxima were found. All methods were calibrated using the standard compounds Trolox (R) and/or gallic acid. Calibration curves were determined (relative standard deviation was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and discussed. Moreover, the data obtained were applied to optimize and to automate all mentioned protocols. Automatic analyzer allowed us to analyse simultaneously larger set of samples, to decrease the measurement time, to eliminate the errors and to provide data of higher quality in comparison to manual analysis. The total time of analysis for one sample was decreased to 10 min for all six methods. In contrary, the total time of manual spectrometric determination was approximately 120 min. The obtained data provided good correlations between studied methods (R = 0.97 - 0.99).
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