Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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LAČNÁ Júlia PŘIKRYL Jan TESHIMA Norio MURAKAMI Hiroya ESAKA Yukihiro FORET František KUBÁŇ Petr

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj Electrophoresis
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://dx.doi.org/10.1002/elps.201900112
Doi http://dx.doi.org/10.1002/elps.201900112
Klíčová slova Capillary electrophoresis; Contactless conductivity detection; Dual detection; Laser induced fluorescence detection; Saliva
Popis In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual (CD)-D-4-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using (CD)-D-4 and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.
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