A new approach of antibodies labeling (NPs) by immunoassay with LA-ICP-MS detection
| Autoři | |
|---|---|
| Rok publikování | 2018 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | TVRDOŇOVÁ, Michaela, Lena ASCHER, Norbert JAKUBOWSKI, Marcela VLČNOVSKÁ, Markéta VACULOVIČOVÁ, Viktor KANICKÝ a Tomáš VACULOVIČ. A new approach of antibodies labeling (NPs) by immunoassay with LA-ICP-MS detection. In European Symposium on Atomic Spectrometry. 2018. |
| Popis | Laser ablation with inductively coupled plasma is still more used in life science as biology and biomedicine and the utilization of metals and proteins determination simultaneously is also growing up. 1,2 We have developed a new strategy of antibody labeling (it can specific binds to proteins) by Au nanoparticles which are composed of thousands of atoms and thus increase the sensitivity enormously and decrease the limit of detection in comparison to lanthanoids labeling. The ability of successfully labelled antibodies bounded to antigen (protein) was proved by dot blot on the membrane and the Au was imaged by LA-ICP-MS. One of the many possible applications is binding of labelled antibodies to the tumour tissues containing metallothionein (known as a transporter of Cu, Zn and heavy metals) and image their distribution in different tumour stage. |
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