Sensitive operation of enzyme-based biodevices by advanced signal processing

Logo poskytovatele
Logo poskytovatele

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

MAZURENKO Stanislav NEVOLOVÁ Šárka KOTLÁNOVÁ Markéta DAMBORSKÝ Jiří PROKOP Zbyněk

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Plos one
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://dx.doi.org/10.1371/journal.pone.0198913
Doi http://dx.doi.org/10.1371/journal.pone.0198913
Klíčová slova SPHINGOMONAS-PAUCIMOBILIS UT26; HALOALKANE DEHALOGENASE; BIOSENSORS; CALIBRATION; SENSORS; EQUATION; TRENDS; TOXINS
Popis Analytical devices that combine sensitive biological component with a physicochemical detector hold a great potential for various applications, e.g., environmental monitoring, food analysis or medical diagnostics. Continuous efforts to develop inexpensive sensitive biodevices for detecting target substances typically focus on the design of biorecognition elements and their physical implementation, while the methods for processing signals generated by such devices have received far less attention. Here, we present fundamental considerations related to signal processing in biosensor design and investigate how undemanding signal treatment facilitates calibration and operation of enzyme-based biodevices. Our signal treatment approach was thoroughly validated with two model systems: (i) a biodevice for detecting chemical warfare agents and environmental pollutants based on the activity of haloalkane dehalogenase, with the sensitive range for bis(2-chloroethyl) ether of 0.01-0.8 mM and (ii) a biodevice for detecting hazardous pesticides based on the activity of y-hexachlorocyclohexane dehydrochlorinase with the sensitive range for y-hexachlorocyclohexane of 0.01-0.3 mM. We demonstrate that the advanced signal processing based on curve fitting enables precise quantification of parameters important for sensitive operation of enzyme-based biodevices, including: (i) automated exclusion of signal regions with substantial noise, (ii) derivation of calibration curves with significantly reduced error, (iii) shortening of the detection time, and (iv) reliable extrapolation of the signal to the initial conditions. The presented simple signal curve fitting supports rational design of optimal system setup by explicit and flexible quantification of its properties and will find a broad use in the development of sensitive and robust biodevices.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info