Detection of antibodies against Borrelia burgdorferi s.l. in wild-type small mammals and comparison of susceptibility of direct methods (PCR and cultivation)
| Autoři | |
|---|---|
| Rok publikování | 2018 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | The aim of this study was to determine the prevalence of antibodies against Borrelia burgdorferi s.l. in wild-type small mammals as well as to compare the sensitivity of direct detection methods (cultivation and PCR). Between 2010 and 2014, 691 wild small mammals were cought in three locations in the Czech Republic. Serum (n = 351) or cardiac rinse (n = 340) were examined with a modified indirect enzyme immunoassay (ELISA). Antibodies against B. burgdorferii s.l. were detected in 12% (484/691) animals. To compare the sensitivity of two direct methods, seventy random samples were used in 2012. B. burgdoferi s.l. was cultured in one sample (1.4%), which was also negative in PCR and ELISA. Using PCR, B. burgdoferi s.l. detected in seven samples (10%), two of them were dubious in ELISA. A total of 23 samples (11 positive and 12 dubious) had antibodies to B. burgdorferi s.l., but were also negative in PCR and cultivation. |
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