Identification of bacterial uropathogens by preparative isoelectric focusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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SALPLACHTA Jiri HORKA Marie RŮŽIČKA Filip SLAIS Karel

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Chromatography A
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.chroma.2017.11.072
Klíčová slova Bacteria; Infection; Isoelectric focusing; Mass spectrometry; Preparative; Urine
Popis This study describes a new method for simultaneous identification of uropathogens in the case of poly bacterial urinary tract infections. The method utilizes recently developed preparative isoelectric focusing (IEF) in cellulose-based separation medium with a subsequent analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Preparative IEF was successfully used for both purification and separation of bacteria, Escherichia coli (pI 4.6) and Staphylococcus aureus (pI 3.4), in urine samples. The focused zones of bacteria, localized by the positions of focused colored pI markers, were easily collected from the separation media after the IEF analysis and then unambiguously identified by MALDI-TOF MS. The proposed method enables the identification of bacteria in urine specimens when the concentration of individual bacteria is >= 10(4) cells mL(-1). Another benefit is the viability of bacteria extracted from the collected fractions after preparative IEF. (C) 2017 Elsevier B.V. All rights reserved.
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