Automated sample preparation for capillary electrophoresis-frontal analysis
Název česky | Automatizovaná příprava vzorků pro kapilární elektroforézu - frontální analýzu |
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Autoři | |
Rok publikování | 2017 |
Druh | Konferenční abstrakty |
Fakulta / Pracoviště MU | |
Citace | |
Přiložené soubory | |
Popis | The capillary electrophoresis-frontal analysis (CE-FA) is commonly used to determined drug-protein binding parameters in their native states without immobilization or labelling. Classical CE-FA sample preparation process uses off-line mixing reagents outside the capillary. On the other hand, as in the case of enzymatic or derivatization reaction, CE can uses electrophoretically mediated microanalysis (EMMA), where the sample is mixed directly in the capillary. This leads to the other advantages such as smaller sample consumption besides full automation. The main objective of this study was to test this methodological approach of sample preparation for CE-FA application. |
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