Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors

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Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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JEŠETA Michal BUDNA Joanna KRANC Wieslawa HANULAKOVA Sarka BRYJA Artur CHACHULA Adrian CIESIÓLKA Sylwia WOJTANOWICZ-MARKIEWICZ Katarzyna BUKOWSKA Dorota BRUSSOW Klaus-Peter BRUSKA Malgorzata NOWICKI Michal ANTOSIK Pawel HULINSKA Pavlina MACHATKOVA Marie ZABEL Maciej KEMPISTY Bartosz

Rok publikování 2017
Druh Článek v odborném periodiku
Časopis / Zdroj Czech Journal of Animal Science
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.17221/90/2016-CJAS
Obor Gynekologie a porodnictví
Klíčová slova pig; ovarian cycle; maturation; developmental competence; folliculogenesis
Popis The zona pellucida proteins belong to a group of proteins that regulate the processes of gamete recognition, interaction, and fusion, since they are recognized as primary and secondary sperm receptors. It is suggested that cortical granule distribution is significantly associated with the zona pellucida structure and membrane preparation to cortical and acrosome reaction. Therefore, this study investigated the zona pellucida marker gene expression (ZP2 and ZP4 protein) in relation to cortical granules distribution in oocytes and puberty status of donors. Oocytes were collected from adult cyclic sows (isolated from medium and small follicles) and juvenile gilts (isolated only from small follicles). The oocytes were examined by RT-qPCR and by confocal microscopy. The expression of genes for ZP2 and ZP4 protein in oocytes collected from small follicles from cycling sows was higher in comparison to oocytes from medium follicles or oocytes collected from small follicles from juvenile gilts (P < 0.001). We also observed increased expression of both ZP2 and ZP4 mRNA in oocytes collected from small follicles (juvenile gilts) as compared to medium follicles (cycling sows) (P < 0.001). Moreover, we found a difference in the distribution of cortical granules. In oocytes from medium follicles, the peripheral localization of cortical granules was twice higher than their central concentration. However, in oocytes derived from small follicles (cyclic sows) cortical granules in comparison to oocytes from medium follicles were more centrally localized (P < 0.05). It has been suggested that the donor puberty status and the size of follicles significantly influenced the zona pellucida gene expression and cortical granule localization in porcine oocytes. This is accompanied by fertilization specificity and fertilizability of porcine oocytes in vitro.
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