Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance

• Autoři: VAULET Lucía Gallo; GRILLOVÁ Linda; MIKALOVÁ Lenka; CASCO Ricardo; FERMEPIN Marcelo Rodríguez; PANDO María A.; ŠMAJS David
• Rok publikování: 2017
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Plos one
• Fakulta / Pracoviště MU: Lékařská fakulta
• Doi: http://dx.doi.org/10.1371/journal.pone.0172905
• Obor: Mikrobiologie, virologie
• Klíčová slova: SYPHILIS-CAUSING STRAINS; RIBOSOMAL-RNA A2058G; AZITHROMYCIN RESISTANCE; SAN-FRANCISCO; UNITED-STATES; TP0548 GENE; PREVALENCE; CHINA; EPIDEMIOLOGY; MUTATIONS
• Popis: A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequencing-based molecular typing (SBMT) and Enhanced CDC-typing (ECDCT). By SBMT, 10 different Treponema pallidum subsp. pallidum (TPA) genotypes were found, of which six were related to the TPA SS14 strain, and four to the TPA Nichols strain. The 23S rRNA gene was amplified in samples isolated from 42 patients, and in six of them (14.3%), either the A2058G (four patients, 9.5%) or the A2059G (two patients, 4.8%) mutations were found. In addition to Taiwan, Madagascar and Peru, Argentina is another country where the prevalence of Nichols-like isolates (26.8%) is greater than 10%.

Související projekty

• Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema
• Molekulární typování původce syfilis v Jižní Americe (Argentinská republika) a v Evropě (Česká republika)
• Studium genomů patogenních treponem na základě analýzy jednotlivých buněk