SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	SILVA Sonia ALTMANNOVÁ Veronika ECKERT-BOULET Nadine KOLESÁR Peter GALLINA Irene HANG Lisa CHUNG Inn ARNERIC Milica ZHAO Xiaolan BURON Line Due MORTENSEN Uffe H. KREJČÍ Lumír LISBY Michael
Rok publikování	2016
Druh	Článek v odborném periodiku
Časopis / Zdroj	DNA Repair
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	http://dx.doi.org/10.1016/j.dnarep.2016.04.001
Obor	Genetika a molekulární biologie
Klíčová slova	Homologous recombination; Rad52; Rad59; Srs2; Rad51; SUMOylation
Popis	Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR. (C) 2016 Elsevier B.V. All rights reserved.
Související projekty:	Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I SUMO a stability genomu