Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

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Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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SILVA Sonia ALTMANNOVÁ Veronika LUKE-GLASER Sarah HENRIKSEN Peter GALLINA Irene YANG Xuejiao CHOUDHARY Chunaram LUKE Brian KREJČÍ Lumír LISBY Michael

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Genes & Development
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1101/gad.276204.115
Obor Genetika a molekulární biologie
Klíčová slova homologous recombination; telomere maintenance; genome integrity; DNA repair; Mph1; Mte1
Popis Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1 Delta mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.
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