TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs

• Autoři: USTIANENKO Dmytro; PASULKA Josef; FEKETOVÁ Zuzana; BEDNAŘÍK Lukáš; ZIGÁČKOVÁ Dagmar; FOŘTOVÁ Andrea; ZAVOLAN Mihaela; VAŇÁČOVÁ Štěpánka
• Rok publikování: 2016
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: EMBO JOURNAL
• Fakulta / Pracoviště MU: Středoevropský technologický institut
• www: http://emboj.embopress.org/content/early/2016/09/19/embj.201694857
• Doi: http://dx.doi.org/10.15252/embj.201694857
• Obor: Biochemie
• Klíčová slova: DIS3L2; RNA surveillance; TSSa; ncRNAs; uridylation
• Popis: Uridylation of various cellular RNA species at the 3' end has been generally linked to RNA degradation. In mammals, uridylated pre-let-7 miRNAs and mRNAs are targeted by the 3' to 5' exoribonuclease DIS3L2. Mutations in DIS3L2 have been associated with Perlman syndrome and with Wilms tumor susceptibility. Using in vivo cross-linking and immunoprecipitation (CLIP) method, we discovered the DIS3L2-dependent cytoplasmic uridylome of human cells. We found a broad spectrum of uridylated RNAs including rRNAs, snRNAs, snoRNAs, tRNAs, vault, 7SL, Y RNAs, mRNAs, lncRNAs, and transcripts from pseudogenes. The unifying features of most of these identified RNAs are aberrant processing and the presence of stable secondary structures. Most importantly, we demonstrate that uridylation mediates DIS3L2 degradation of short RNA polymerase II-derived RNAs. Our findings establish the role of DIS3L2 and oligouridylation as the cytoplasmic quality control for highly structured ncRNAs.

Související projekty

• Funkční a biochemická charakterizace proteinu Dis3L2, třetího lidského homologu hlavní kvasinkové exosomové nukleázy Dis3p
• Studium úlohy uridylace RNA v lidských buňkách
• CEITEC 2020