Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode

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TŘÍSKOVÁ Iveta GURECKÝ Libor TRNKOVÁ Libuše

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Monatshefte für Chemie - Chemical Monthly
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://dx.doi.org/10.1007/s00706-016-1689-7
Doi http://dx.doi.org/10.1007/s00706-016-1689-7
Obor Fyzikální chemie a teoretická chemie
Klíčová slova DNA heptamers; hanging mercury drop electrode; linear sweep voltammetry; elimination voltammetric procedure; adsorption isotherm; adsorption coefficient
Popis The focus of the presented study is electrochemical investigation of DNA heptamers d(GCNNNGC) with a different center trinucleotide sequence (NNN = GAA, AAA, CCC and GGG, where A, C and G is adenine, cytosine and guanine, respectively). In phosphate-acetate buffer at pH 5.8 all heptamers were adsorbed on a hanging mercury electrode and further reduced and oxidized. The voltammetric reduction peaks of A and C moiety and oxidation peaks of G moiety at different concentrations (0.25 to 10 µmol•L-1) were analyzed. It was found that the changes in the central trinucleotide sequence of heptamers result in altered adsorption ability at a charged phase interface. An electron transfer process in an adsorptive state was evidenced by the elimination voltammetric procedure (EVP). For the evaluation of adsorption efficiency of heptamers the Langmuir adsorption model was applied and the adsorption coefficient b was calculated.
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