Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella
| Autoři | |
|---|---|
| Rok publikování | 2016 |
| Druh | Článek ve sborníku |
| Konference | XVI. Workshop of Physical Chemists and Electrochemists |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Biochemie |
| Klíčová slova | immunosensor; electrochemical impedance spectroscopy; food safety; Salmonella Typhimurium |
| Popis | A label-free biosensor based on electrochemical impedance spectroscopy (EIS) was developed for the detection of Salmonella Typhimurium in milk. Specific antibody was immobilized on a screen-printed electrode, the electrode was incubated directly with the sample and binding of bacteria was measured as a change of impedance. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy (AFM) was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. A wide linear response was obtained in the range between 10^3 and 10^8 CFU·mL-1. |
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