cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase

Logo poskytovatele
Logo poskytovatele

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
Autoři

DOKLÁDAL Ladislav HONYS David RANA Rajiv LEE Lan-Ying GELVIN Stanton B. SÝKOROVÁ Eva

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Frontiers in Plant Science
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://journal.frontiersin.org/article/10.3389/fpls.2015.00985/full
Doi http://dx.doi.org/10.3389/fpls.2015.00985
Obor Botanika
Klíčová slova telomerase; nuclear poly(A)-binding protein; telobox; metallothionein 2A; MODIFIER OF snc1; putative nuclear DNA-binding protein G2p; oxidation-related zinc finger 2 protein; BiFC
Popis Telomerase-reverse transcriptase (TERT) plays an essential catalytic role in maintaining telomeres. However, in animal systems telomerase plays additional non-telomeric functional roles. We previously screened an Arabidopsis cDNA library for proteins that interact with the C-terminal extension (CTE) TERT domain and identified a nuclearlocalized protein that contains an RNA recognition motif (RRM). This RRM-protein forms homodimers in both plants and yeast. Mutation of the gene encoding the RRM-protein had no detectable effect on plant growth and development, nor did it affect telomerase activity or telomere length in vivo, suggesting a non-telomeric role for TERT/RRM-protein complexes. The gene encoding the RRM-protein is highly expressed in leaf and reproductive tissues. We further screened an Arabidopsis cDNA library for proteins that interact with the RRM-protein and identified five interactors. These proteins are involved in numerous non-telomere-associated cellular activities. In plants, the RRM-protein, both alone and in a complex with its interactors, localizes to nuclear speckles. Transcriptional analyses in wild-type and rrm mutant plants, as well as transcriptional co-analyses, suggest that TERT, the RRM-protein, and the RRM-protein interactors may play important roles in non-telomeric cellular functions.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info