Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	MLYNÁRIKOVÁ Katarína SAMEK Ota BERNATOVÁ Silvie RŮŽIČKA Filip JEŽEK Jan HÁRONIKOVÁ Andrea ŠILER Martin ZEMÁNEK Pavel HOLÁ Veronika
Rok publikování	2015
Druh	Článek v odborném periodiku
Časopis / Zdroj	Sensors
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	http://dx.doi.org/10.3390/s151129635
Obor	Mikrobiologie, virologie
Klíčová slova	Raman spectroscopy; bacteria; yeasts; culture media
Popis	Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans). Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar) and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.
Související projekty:	Zapojení České republiky do aktivit Americké společnosti pro mikrobiologii (ASM)