Characterization of Staphylococcus aureus strains isolated from Czech cystic fibrosis patients: high rate of ribosomal mutation conferring resistance to MLSB antibiotics as a result of long-term and low-dose azithromycin treatment

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Publikace nespadá pod Fakultu sportovních studií, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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TKADLEC Jan VAŘEKOVÁ Eva PANTŮČEK Roman DOŠKAŘ Jiří RŮŽIČKOVÁ Vladislava BOTKA Tibor FILA Libor MELTER Oto

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Microbial Drug Resistance
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://online.liebertpub.com/doi/10.1089/mdr.2014.0276
Doi http://dx.doi.org/10.1089/mdr.2014.0276
Obor Mikrobiologie, virologie
Klíčová slova Staphylococcus aureus; cystic fibrosis; small colony variants; MLSB resistance;
Přiložené soubory
Popis Staphylococcus aureus is one of the most frequent pathogens infecting the respiratory tract of patients with cystic fibrosis (CF). This study was the first to examine S. aureus isolates from CF patients in the Czech Republic. Among 100 S. aureus isolates from 92 of 107 observed patients, we found a high prevalence of resistance to macrolide–lincosamide–streptogramin B (MLSB) antibiotics (56%). More than half of the resistant strains (29 of 56) carried a mutation in the MLSB target site. The emergence of MLSB resistance and mutations conferring resistance to MLSB antibiotics was associated with azithromycin treatment (p=0.000000184 and p=0.000681, respectively). Methicillin resistance was only detected in 3% of isolates and the rate of resistance to other antibiotics did not exceed 12%. The prevalence of small-colony variant (SCV) strains was relatively low (9%) and eight of nine isolates with the SCV phenotype were thymidine dependent. The study population of S. aureus was heterogeneous in structure and both the most prevalent community-associated and hospital-acquired clonal lineages were represented. Of the virulence genes, enterotoxin genes seg (n=52), sei (n=49), and sec (n=16) were the most frequently detected among the isolates. The PVL genes (lukS-PV and lukF-PV) have not been revealed in any of the isolates.
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