Sex differences in the reinstatement of methamphetamine seeking after forced abstinence in Sprague-Dawley rats

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Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
Název česky Mezipohlavní rozdíly ve vyhledávání metamfetaminu po nucené abstinenci u Sprague-Dawley potkana
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RUDÁ Jana AMCHOVÁ Petra BABINSKÁ Zuzana DUŠEK Ladislav MICALE Vincenzo ŠULCOVÁ Alexandra

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Frontiers in Psychiatry
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.3389/fpsyt.2015.00091
Obor Farmakologie a lékárnická chemie
Klíčová slova methamphetamine; reinstatement of drug-seeking behavior; forced abstinence; sex/gender differences; Sprague-Dawley rats
Přiložené soubory
Popis Preventing relapse to drug abuse is one of the struggles faced by clinicians in order to treat patients with substance use disorders (DSM-5). There is a large body of clinical evidence suggesting differential characteristics of the disorder in men and women, which is in line with preclinical findings as well. The aim of this study was to assess differences in relapse-like behavior in methamphetamine (METH) seeking after a period of forced abstinence, which simulates the real clinical situation very well. Findings from such study might add new insights in gender differences in relapse mechanisms to previous studies, which employ a classical drug or cue-induced reinstatement procedure following the extinction training. Adult male and female Sprague-Dawley rats were used in IV self-administration procedure conducted in operant boxes using nose-poke operandi (Coulborn Instruments, USA). Active nose-poke resulted in activation of the infusion pump to deliver one intravenous infusion of METH (0.08 mg/kg). After baseline drug intake was established (maintenance phase), a period of forced abstinence was initiated and rats were kept singly in their home cages for 14 days. Finally, one reinstatement session in operant boxes was conducted. Females were found to self-administer significantly lower dose of METH. The relapse rate was assessed as a number of active nose-pokes during the reinstatement session, expressed as a percentage of active nose-poking during the maintenance phase. Females displayed approximately 300% of active nose-pokes compared to 50% in males. This indicates higher vulnerability to relapse of METH seeking behavior in female rats. This effect was detected in all females, independently of current phase of their estrous cycle. Therefore, this paradigm using operant drug self-administration and reinstatement of drug-seeking after forced abstinence model can be used for preclinical screening for potential new anti-relapse medications specific for women.
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