Haematopoietic developmental potential of human pluripotent stem cell lines

Logo poskytovatele
Logo poskytovatele

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Fakultu informatiky. Oficiální stránka publikace je na webu muni.cz.
Autoři

TESAŘOVÁ Lenka ŠIMARA Pavel STEJSKAL Stanislav KRONTORÁD KOUTNÁ Irena

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Folia biologica
Fakulta / Pracoviště MU

Fakulta informatiky

Citace
Obor Genetika a molekulární biologie
Klíčová slova human pluripotent stem cells; hematopoietic development; embryoid bodies; CD34+ cells
Popis The generation of hematopoietic progenitors from human pluripotent stem cells (hPSCs) presents great promise for cell-replacement therapies. However, current protocols for hematopoietic differentiation of hPSCs suffer from low efficiency and functional defects in the derived cells. The technology is also limited by variable ability of hPSC lines to generate blood cells in vitro. To address this issue, methodologies for hematopoietic differentiation in feeder-free conditions were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines in this study. It was found that these cell lines did not generate hematopoietic progenitors in such an extent as H1 and H9 hESC lines used for this purpose in the vast majority of relevant studies. These results suggest that for clinical application of blood cells derived from hPSCs, possibly from autologous hiPSCs, it is necessary to overcome the variability in the hematopoietic developmental potential of individual hPSC lines.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info