Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling
Autoři | |
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Rok publikování | 2013 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Chemical communications |
Fakulta / Pracoviště MU | |
Citace | |
www | http://www.rsc.org/chemcomm |
Doi | http://dx.doi.org/10.1039/C3CC41438H |
Obor | Biochemie |
Klíčová slova | DNA polymerase; DNA labelling; oligonucleotides |
Popis | Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template. |
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