Tumor necrosis factor alpha and its receptors upregulation in dorsal root ganglia of rheumatoid arthritis model
| Název česky | Zvýšená produkce tumor necrosis factor alpha a jeho receptorů ve spinálním gangliu u modelu revmatoidní artritis |
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| Autoři | |
| Rok publikování | 2013 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Introduction: Rheumatoid arthritis (RA) is a chronic, autoimmune inflammatory disease that affects approximately 1% of the population. Tumor necrosis factor-a TNF-a, that are also important in pathogenesis of RA, binds to two receptors, the type 1 TNF receptor (p55, TNFR1) and the type 2 TNF receptor (p75, TNFR2), that are expressed on many types of the cells. The aim of our present study was to compare a distribution of TNF-a and its receptors, TNFR1 and TNFR2, in the lumbar dorsal root ganglia (DRGs) and changes of their amount in time. Methods: We used a rat model of unilateral antigen-induced arthritis (AIA). For induction of AIA, rats (n=14) were immunized by an intra-articular injection of antigen [500 ug methylated bovine serum albumin (m-BSA)] in saline emulsified with 500 ul of complete Freund’s adjuvant into the knee joint. The knee joints of control rats were injected by 500 ul of saline and the naive rats were without AIA induction and injection of saline. All animals were left to survive for 1, 2, 4, 14 and 21 days. In our experiments, we used immunofluorescence detection and Western blot analysis. Results: TNFa, TNFR1 and TNFR2 were found bilaterally in lumbar L3 and L5 DRGs. One day after induction of AIA, we observed robust bilaterally increasing of TNFa and its receptors in both investigated ganglia. In contrast, from two to fourteen days after immunization, we found almost no reactions in the all observed ganglia when compared to control and naive rats. Twenty one days after AIA, i.e. during the chronic phase, we observed again bilateral increasing of TNFa, TNFR1 and TNFR2. In summary, after induction of RA with Complete Freund Adjuvans we observed bilateral increasing of TNFa and its receptors into the lumbar DRGs. Conclusion: The present study has shown that AIA is associated with a bilateral invasion of TNFa and its receptors, TNFR1 and TNFR2, into investigated ganglia correlated with the sinusoid which corresponds with the clinical stages of the RA. |
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