A determination of oxidative stress in tumour cells treated with cytostatics
| Autoři | |
|---|---|
| Rok publikování | 2012 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Oxidative stress as a response to anticancer drugs is not sufficiently investigated and direct measurement of reactive oxygen species and oxidative stress markers in clinical medicine is still difficult. In our study, oxidative status was studied in model tumour cell lines derived from tumours of the prostate (PC-3, PNT1A, 22RV1). Cell lines were cultured with anticancer drugs carboplatin, cisplatin, oxaliplatin at concentrations of 0, 10, 30 , 50, 80, 100, 150, 180, 200 and 250 micromol/l and drugs as doxorubicin and ellipticine at concentrations of 0, 0.5, 1, 1.5, 2, 4, 6, 10, 12, 15 micromol/l. The rate of oxidative stress was determined by antioxidant activity (FRAP, DPPH and ABTS). Based on the results obtained from oxidative changes in the cells determined by FRAP and ABTS, it was found the lowest antioxidant activity as 0.06 mg of Trolox/l was in cell lines 22RV1 treated with carboplatin at a concentration of 100 micromol/l. PNT1A cells gave the lowest antioxidant activity of 0.35 mg of Trolox/l after exposure to cisplatin concentration of 100 micromol/l. |
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