Electrochamical characterization of doxorubicin interaction with DNA
| Autoři | |
|---|---|
| Rok publikování | 2012 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Doxorubicin as a member of anthracycline antibiotics is one of the most widely used for the treatment of malignant tumours with the ability to i11teract with DNA. Electrochemistry is considered one of the most sensitive methods for studying DNA. The aim of the study was: i) electrochemical characterization of the interaction of doxorubicin with single and double stranded oligonucleotides. ii) investigation of the influence of different sequence on the interaction. Hi) time dependence of the effect of doxorubicin and oligonucleotides. Four different variants of ODN were designed and the influence of sequence on interaction with ctoxorubicin was investigated. The obtained data were processed showing the correlation dependence of size change of DOXO peak to peak size change of the peak of each ODN. This dependence was studied also in the time manner (time interaction DOXO with ODN) as 30, 150, 240 and 300 min. The value of (100,0) represents the initial state of electrochemical analysis. This puint must be understood as a starting point for all four of the oligonucleotides (MT5, GL6, GIA and CA3). When comparing the results for three ratios of the mixture between drug and ssODN and/or dsODN we found that the interval of peak intensities of oligonucleotide reduced with the increasing ratio of oligonucleotide doxorubicin, i.e. for ratio 1:1 it is 25-85 %, for ratio 1:2 it is 16-60 % and for ratio 1:5 it is 19-51 %. The interval of relative signal intensities of doxorubicin ranges from 8 to 100 % for ratio 1:1, 22-100 % for ratios 1:2 and 1:5. The results showed the assumption that DOXO peak height increased with increasing concentration of the drug intercalated into ssODN and dsODN. |
| Související projekty: |