Project information
Charakterizace MAGE proteinů: kofaktory E3 ubiquitin ligáz?
- Project Identification
- IAA501630902
- Project Period
- 1/2009 - 12/2011
- Investor / Pogramme / Project type
-
Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
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Central European Institute of Technology
- doc. Mgr. Jan Paleček, Dr. rer. nat.
- Mgr. Sreenivas Reddy Bathula, Ph.D.
- Mgr. Lucie Bozděchová, Ph.D.
- Marc Guérineau, Ph.D., M.Sc.
- Mgr. Tomáš Klumpler, Ph.D.
- doc. RNDr. Jaromír Marek, Ph.D.
- prof. RNDr. Vladimír Sklenář, DrSc.
- Mgr. Ondrej Šedo, Ph.D.
- Mgr. Kateřina Zábrady, Ph.D.
- prof. RNDr. Zbyněk Zdráhal, Dr.
- prof. Mgr. Lukáš Žídek, Ph.D.
- Keywords
- MAGE (melanoma antigen) proteins; protein structure and interactions; RING finger proteins; E3 ubiquitin ligases; DNA repair; chromatin structure; SMC5-6 complex
- Cooperating Organization
-
The University of Sussex
- Responsible person Prof. Alan R. Lehmann
The MAGE (Melanoma antigen) family consists of 35 proteins in humans that contain a "MAGE homology domain" (MHD) but have widely differing functions. The Smc5-6 complex, which functions in DNA repair, consists of MAGEG1-Nse1 heterodimer. Taking, Nse1 is a putative RING finger E3 ubiquitin ligase, there are two other reports of MAGE proteins interacting with RING-finger proteins. In addition, our structure predictions suggest that part of MHD has similarities to cullin components of E3 ligases. We will test the hypothesis that MAGE proteins are cofactors in E3 ligase complexes. We will carry out structural analyses of the MHD fold using NMR spectroscopy and X-ray crystallography to test our structural predictions. We will mutate critical residues in MAGEG1, that we predict to interact with Nse1, to see if this specifically destroys this interaction and affects DNA repair. We will search for and analyze new RING partners interacting with MAGE proteins to find out MHD common features.
Publications
Total number of publications: 11
2015
-
The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
Cell Cycle, year: 2015, volume: 14, edition: 6, DOI
2013
-
Evolution and function of non-SMC subunits of SMC5/6 complex
Year: 2013, type: Conference abstract
2012
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Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins
PLoS One, year: 2012, volume: 7, edition: 4, DOI
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MAGE proteiny jako kofaktory buněčných procesů
XII. mezioborové setkání mladých biologů, biochemiků a chemiků z Čech a SLovenska, year: 2012
2011
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CHARACTERIZATION OF THE NSE4/EID BINDING TO NSE3/MAGE PROTEINS
Year: 2011, type: Conference abstract
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Interactions between the Nse3 and Nse4 Components of the SMC5-6 Complex Identify Evolutionarily Conserved Interactions between MAGE and EID Families
PloS ONE, year: 2011, volume: 6, edition: 2, DOI
2010
-
The MAGE and EID proteins affect transcription regulation of target genes
Year: 2010, type: Conference abstract
-
The Nse3/MAGE and Nse4/EID proteins are involved in transcription regulation
Year: 2010, type: Conference abstract
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The NSE3/MAGE interactions with NSE4/EID proteins are evolutionarily conserved
Year: 2010, type: Conference abstract