Project information
High-resolution cytometry of living cells
- Project Identification
- GA202/04/0907
- Project Period
- 1/2004 - 12/2006
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- structure of the cell,living cells,analytic cytometry
- Cooperating Organization
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Institute of Biophysics
- Responsible person doc. RNDr. Stanislav Kozubek, DrSc.
In the post-genomic era, the structure of the cell nucleus has become the key to understanding the most important cellular processes such as cell
differentiation, apoptosis or transformation. In order to investigate highly organized nuclear structure, methods of high-resolution cytometry developed in our laboratory in combination with techniques that enable visualization of nuclear structures in living cells will be used. DNA constructs coding fusion proteins will be employed to study DNA-protein interaction in vivo. As a model system proteins related to acute
promyelocytic leukemia will be used. Nuclear localization of PML, RARa, PML/RARa tagged to GFP, RFP and YFP will be investigated in PR-9 cells in which the PML/RARa protein can be induced by increased concentration of Zn ions. Interaction with DNA will be studied using subsequent 3D FISH in cells previously investigated in vivo. For both in vivo studies and FISH the technology of high-resolution cytometry will be used. Automated image acquisition, on-line analysis, storage and evaluation of the arrays of parameters in computer memory will be used. The main objective of the project will be the introduction of the methodology of GFP techniques in our laboratory and its combination with high-resolution cytometry. Substantially deeper understanding of the functional topography of proteins related to APL is expected. The long-term practical goal of the project will be the use of experimental results and technology developed in our laboratories in diagnostics and therapy of cancer.
Results
Publications
Total number of publications: 22
2005
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Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
Year: 2005, type: Conference abstract
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Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases
Journal of Cell Science, year: 2005, volume: 118, edition: 21
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Sledování změn genové exprese u pacientů s kolorektálním karcinomem pomocí cDNA microarrays technologie a jejich diagnostika pomocí transkripčních map
Year: 2005, type: Conference abstract
2004
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Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy
Microscopy research and technique, year: 2004, volume: 64, edition: 2
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Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images
Biophysics of the Genome, year: 2004
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Cytological study of DNA methylation and histone methylation patterns in human cell lines
Biophysics of the Genome, year: 2004
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From Jenalumar to Ultrafast Microscopy of Living Cells
Biophysics of the Genome, year: 2004
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Gene expression in colorectal carcinoma determined by cDNA microarrays using a combination of several evaluation approaches.
Biophysics of the Genome, year: 2004
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Image analysis of the movement of sub-cellular structures
Biophysics of the Genome, year: 2004
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In vivo imaging of cell interior using automated high-resolution cytometry
Biophysics of the Genome, year: 2004