Problematic glycosyltransferase Rv3782 from Mycobacterium tuberculosis and its production in new expression system
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| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Mycobacterium tuberculosis is germ causing one of the most difficultly treated and in many cases lethal infectious disease – tuberculosis. This pathogenic bacterium is characterized by unique cell envelope – dominant feature of M. tuberculosis and other mycobacterial pathogens and crucial factor of virulence and resistance against toxic insult, such as antibiotics and components of the macrophage's bactericidal arsenal. The synthesis of this mycobacterial cell wall is mediated by dominating group of enzymes – glycosyltransferases. Rv3782 is mycobacterial glycosyltransferase which is responsible for synthesis of mycobacterial galactan - very important element of mycobacterial cell wall and its lack can cause growth inhibition up to death. Due to its role in the cell wall synthesis, the Rv3782 galactofuranosyltransferase may represent very attractive target for new drug development against tuberculosis. In our laboratory there was created new expression system pMTW for expression of problematic proteins such as mycobacterial glycosyltransferases, because these proteins are unstable, often membrane-associated and present in the cell in very low concentration. |
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