Determination of amino acids by capillary electrophoresis using reversed electro-osmotic flow: a critical evaluation
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Year of publication | 2012 |
Type | Conference abstract |
MU Faculty or unit | |
Citation | MÁDR, Aleš, Kateřina SVOBODOVÁ and Zdeněk GLATZ. Determination of amino acids by capillary electrophoresis using reversed electro-osmotic flow: a critical evaluation. In Advances in Chromatography and Electrophoresis & Chiranal 2012. 2012. ISBN 978-80-244-3115-4. |
Description | Capillary electrophoresis with contactless conductivity detection (CCD) is useful combination for an analysis of the polar compounds with no chromophores in the structure. Using conventional UV/Vis spectrophotometric detection, derivatization of the analytes is needed. When CCD is used, derivatization of a sample can be omitted. However, it is a little bit challenging to keep a signal-to-noise ratio in a suitable range for the analytical purposes, when complex buffers are employed. The case is subject of this contribution. |
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