Novel prostate cancer tumour markers in a cell line model
| Authors | |
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| Year of publication | 2011 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | To date, it is not possible to differentiate between high-risk and latent forms of prostate cancer. The aim of this study is to determine RNA and protein level of potential tumour markers in the cell lines PNT1A and 22Rv1, which represent healthy prostate tissue and high-grade prostate cancer. We determined significantly decreased level of caveolin-1 on both RNA and protein level, which is in contradiction with our previous findings regarding to its serum level in prostate cancer patients (found increased in high-grade tumours). We also determined increased level of metallothionein on RNA and protein level in tumorous tissue, which is in agreement with previous studies focused on determination of metallothionein in serum (where increased levels were observed). Moreover, we determined significantly up-regulated RNA level of zinc(II) transporters ZIP-1 and ZnT-1. Regarding ZnT-1, such changes of expression have not been described yet. These data suggest that combination of caveolin-1, metallothionein and ZIP-1 or ZnT-1 may be utilized as a tool to distinguish aggressive forms of prostate cancer from clinically latent forms. It is expected, that such diagnostic tool is to be used for the evaluation of bioptical samples, however, verification in a large follow-up set of samples is needed. |