Comparison of Two Electrophoretic Methods Documented by Separation of Dextromethorphan and its Metabolites
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| Year of publication | 2011 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Capillary electrophoresis (CE) belongs to powerful analytical techniques and its great advantage is the existence of various modes. It allows analyses of different compounds from inorganic particles to biopolymers. CE is applied to drug assays because of its unique separation mechanism, speed, efficiency and versatility. Although capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography are the most commonly used modes for drug analysis, the usage of others (e.g. NACE, ITP, CEC) is increasing. In our work we focused on the comparison of CZE and NACE methods applied to determination of a drug and its metabolites. As a model drug dextromethorphan was chosen. Parameters for CZE method were taken from the literature and conditions for NACE were based on the publication. Separations in both modes were carried out under these conditions: a fused silica capillary with inner diameter of 75 um, 48,5/40 cm Ltot/Leff, the positive separation voltage and UV detection at 200 nm. The methods were compared based on the following parameters: repeatability of migration times, normalized peak areas, peak resolution and total analysis time. Other parameters such as background electrolyte, sample matrix and working conditions will be discussed. |
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