Enhancement of AhR-mediated activity of selected pollutants and their mixtures after interaction with dissolved organic matter

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Authors

BITTNER Michal MACÍKOVÁ Petra GIESY John P. HILSCHEROVÁ Klára

Year of publication 2011
Type Article in Periodical
Magazine / Source Environment International
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1016/j.envint.2011.03.016
Field Environment influence on health
Keywords Humic substances; AhR; Mixture; Interaction; H4IIE-luc; Organic pollutants
Description In vitro effects of binary mixtures of various DOM (mainly HS) with various HOCs on AhR-mediated responses were studied by use of H4IIE-luc cells. 6 out of 12 DOM activated the AhR even at environmentally relevant concentrations (17 mg L(-1)). In simultaneous exposures of H4IIE-luc cells to DOM and each of the model compounds, 2,3,7,8-TCDD, PCB126, PCB169, benzo[a]pyrene, benzo[a]anthracene, dibenz[a,h]anthracene, fluoranthene, a mixture of POPs, a mixture of PAHs, and a mixture of all HOCs, either significant additive or facilitative effects were observed when compared to activities of single HOCs. No significant decrease of effects due to sorption of HOCs to DOM was observed, even in subsequent experiments when HOCs + DOM mixtures were preincubated for six days before exposure to H4IIE-luc. Thus, DOM does not seem to protect organisms against AhR-mediated toxic effects of HOCs (as predicted due to sorption of HOCs on DOM), but it can enhance their potency for AhR-mediated effects in some situations.
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