ANALYTICAL ULTRACENTRIFUGATION AS AN EFFECTIVE TOOL FOR IDENTIFICATION OF PROTEIN OLIGOMERIC STATE

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Authors

ROZUMOVÁ Petra KOMÁREK Jan HOUSER Josef WIMMEROVÁ Michaela

Year of publication 2011
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Analytical ultracentrifugation is a powerful technique for characterization of macromolecules in solution with wide applications in biomolecular research. This method can be used to examine purity of the sample, detect conformation changes, analyze associating systems or determine molecular weights of macromolecules. The contribution is focused on application of sedimentation velocity experiments in the study of proteins and provides few examples of usefulness of the method.
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