A new approach how to define coefficient of electroactivity of adenine and its twelve derivatives using flow injection analysis with amperometric detection.
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | Electroanalysis |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=17&SID=V254NakJe2LpBEPl4g1&page=1&doc=1 |
| Doi | http://dx.doi.org/10.1002/elan.201100064 |
| Field | Physical chemistry and theoretical chemistry |
| Keywords | Adenine derivatives; Flow injection analysis; Amperometric detection; Glassy carbon electrode; Electroactivity |
| Description | We studied the electrochemical behaviour of adenine derivates (adenosine, 2-aminopurine, 2,6-diaminopurine, 6-benzyl-aminopurine, adenosine monophosphate, cyclic adenosine monophosphate, nicotinamide adenine dinucleotide, adenosine triphosphate, S-adenosyl-L-methionine, and synthetic derivatives AD-3, AD-6 and AD-9) using flow injection analysis with electrochemical detection using a glassy carbon electrode. The influences of pH, flow rate and potential on the signal height of the studied derivates were tested. The optimal pH was 3, the flow rate of the mobile phase 0.75 mLmin(-1) and the potential 1100 mV. Further, we attempted to characterize each of the studied derivatives by mathematical equations and classic analytical parameters. The lowest detection limit was estimated for adenine as 0.9 nM and 2-aminopurine as 0.5 nM. |
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