Substrate Specificity of Haloalkane Dehalogenases

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Authors

KOUDELÁKOVÁ Táňa CHOVANCOVÁ Eva BREZOVSKÝ Jan MONINCOVÁ Marta FOŘTOVÁ Andrea JARKOVSKÝ Jiří DAMBORSKÝ Jiří

Year of publication 2011
Type Article in Periodical
Magazine / Source Biochemical Journal
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1042/BJ20101405
Field Biochemistry
Keywords substrate specificity; haloalkane dehalogenases; halogenated compounds
Description An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate specificity data, and analysis using multivariate statistics. We describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. The enzymes were characterized experimentally using a set of 30 substrates selected using statistical experimental design from a set of nearly 200 halogenated compounds.
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