Substrate Specificity of Haloalkane Dehalogenases
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | Biochemical Journal |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1042/BJ20101405 |
| Field | Biochemistry |
| Keywords | substrate specificity; haloalkane dehalogenases; halogenated compounds |
| Description | An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate specificity data, and analysis using multivariate statistics. We describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. The enzymes were characterized experimentally using a set of 30 substrates selected using statistical experimental design from a set of nearly 200 halogenated compounds. |
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