APTAMER BASED CD-SENSORS FOR THROMBIN RECOGNITION BY MEANS OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY

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Authors

CASTILLO Gabriela TRNKOVÁ Libuše FOGUEL Marcos Vinicius HIANIK Tibor

Year of publication 2011
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Biosensors based on DNA or RNA aptamers, represent a very efficient platform for protein recognition1. We developed a novel label-free aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein complex formation using gold CD-trodes (GCDT). GCDT are electrodes fabricated from recordable compact discs that contain a fine gold layer on their surface. Low-cost GCDTs present good versatility and acceptable reproducibility. The thrombin biosensor is constructed by self-assembling a thiol-modified aptamer onto a GCDT surface, followed by recognition of the protein via monitoring the interfacial electron transfer resistance in the presence of an [Fe(CN)6]3-/4- redox couple2,3. The aptasensor was also characterized by cyclic voltammetry and the individual binding steps were analysed by scanning electron microscopy.
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