Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | International Journal of Laboratory Hematology |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | microchimerism; chimerism; real-time PCR; relapse; acute myeloid leukemia |
| Description | Using real-time PCR (RQ-PCR), we compare peripheral blood samples obtained from patients with acute myeloid leukemia (AML) before hematological relapse and those taken during complete remission. By comparison of these two groups, we describe microchimerism dynamics clearly connected with imminent AML relapse. Mere reappearance of autologous hematopoiesis within patients with complete donor chimerism is alarming, and another sample with further increase confirms ongoing relapse. In case of patients with continuous microchimerism, another two consecutive samples with increasing trend are required. RQ-PCR predicted a significantly higher number of hematological relapses (87% vs. 39%) with a median anticipation period of 33 days, 26 days earlier than conventional PCR (P = 0.0002). Moreover, the outcome of microchimerism dynamics was in complete agreement with monitoring of minimal residual disease when analyzed from the same cell compartment. |
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