Evaluation of cytotoxic activity of Schisandra chinensis lignans

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Authors	SMEJKAL Karel SLAPETOVÁ Tereza KRMENČÍK Pavel BABULA Petr DALL'ACQUA Stefano INNOCENTI Gabbriella VANČO Jan CASARIN Elisabetta CARRARA Maria KALVAROVÁ Karolina DVORSKÁ Margita SLANINA Jiří KRAMÁŘOVÁ Eva JULÍNEK Ondřej URBANOVÁ Marie
Year of publication	2010
Type	Article in Periodical
Magazine / Source	Planta Medica
MU Faculty or unit	Faculty of Medicine
Citation
Field	Pharmacology and pharmaceutical chemistry
Keywords	Schisandra chinensis; Schisandraceae; dibenzocyclooctadiene lignans; cytotoxicity; apoptosis
Description	Using exhaustive chromatographic separation we have isolated (-)-tigloyl-deangeloyl-gomisin F as a novel dibenzocyclooctadiene lignan from schisandra chinensis. With the help of HPLC, we further isolated (+)-schisandrin, (+)-deoxyschisandrin, (+)-gamma-schisandrin, (-)-gomisin J, (+)-gomisin A, (-)-gomisin N, (-)-tigloyl-gomisin P, (-)-wuweizisu C, (-)-gomisin D, rubrisandrin A, (-)-gomisin G, (+)-gomisin K (3) and (-)-schisantherin C. A full NMR description of (-)-schisantherin C was carried out with the aim to confirm previous reports of its structure. Compounds isolated were identified on the basis of UV, IR, (1)H- and (13)C-NMR and MS. The cytotoxicity of lignans was tested for the BY-2 cell line alone and as a synergistic effect with the cytotoxic agent camptothecin. Lignans showed various toxicity and synergistic and antagonistic effects on camptothecin-induced cytotoxicity. Cytotoxicity against colon cancer cell line LoVo was also tested.
Related projects:	Regulace biosyntézy sekundárních metabolitů v buněčné kultuře Schisandra chinensis