Assessment of adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) mRNA expression in patients with de novo chronic myelogenous leukemia: the role of different cell types.

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Authors	RÁČIL Zdeněk RÁZGA Filip MACHOVÁ POLÁKOVÁ Kateřina BUREŠOVÁ Lucie POLÍVKOVÁ Václava DVOŘÁKOVÁ Dana ŽÁČKOVÁ Daniela KLAMOVÁ Hana CETKOVSKÝ Petr MAYER Jiří
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Leukemia & Lymphoma
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.3109/10428194.2010.533220
Field	Oncology and hematology
Keywords	mRNA exprese; CML
Description	In this study, we investigated differences in ABCB1 mRNA expression measured in peripheral blood (PB) leukocytes (LEU) as well as polymorphonuclear (PMNCs) and mononuclear cells (MNCs) of PB LEU obtained from healthy volunteers and patients with de novo CML. In addition, we analyzed the relationship between the percentage of individual cells that comprise the total LEU count and ABCB1 mRNA expression assessed from the total LEU. We have shown that ABCB1 mRNA transcript levels are significantly higher in PB MNCs than in PB PMNCs and are lower in patients with de novo CML compared to healthy individuals. Moreover, we have demonstrated the importance of the cell composition of analyzed samples in ABCB1 mRNA expression analysis.
Related projects:	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies