Reciprocal and multi-species chromosome BAC painting in crucifers (Brassicaceae)

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Authors

LYSÁK Martin MANDÁKOVÁ Terezie LACOMBE Eric

Year of publication 2010
Type Article in Periodical
Magazine / Source Cytogenetic and Genome Research
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Arabidopsis halleri; Arabidopsis thaliana; BAC FISH; Brassicaceae; Collinearity; Comparative chromosome painting; Plant cytogenetics
Description The paper reports on principles of multi-species and multi-directional painting in crucifer species (Brassicaceae). We have successfully tested chromosome-specific BAC contigs of A. thaliana (n = 5) and A. halleri (n = 8) as probes for reciprocal BAC painting. Furthermore,BAC contigs of both Arabidopsis species were applied as multi-species painting probes to a third crucifer species, Noccaea caerulescens (n = 7), revealing their shared chromosome homeology. Specifically, we found homeology across portions of chromosomes At2, Ah4, and Nc4, which reflects their shared common origin with chromosome AK4 of the Ancestral Crucifer Karyotype (n = 8). We argue that multispecies and multi-directional painting will significantly expedite comparative cytogenomics in Brassicaceae and other plant families.
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