125I-labelled iodothyronines: useful tools for studies of effects of an antidepressant drug fluoxetine in the rat

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Authors	PAVELKA Stanislav
Year of publication	2010
Type	Article in Periodical
Magazine / Source	Journal of Radioanalytical and Nuclear Chemistry
MU Faculty or unit	Faculty of Science
Citation
Field	Pharmacology and pharmaceutical chemistry
Keywords	Antidepressant drug; radiometric enzyme assay; thyroid hormone
Description	We followed the effects of an antidepressant drug fluoxetine (Fluox), administered sub-chronically to Wistar rats by itself, or in combination with 3,3',5-triiodothyronine. In studies of the interaction of Fluox with the metabolism of thyroid hormones, we applied adapted radiometric enzyme assays for iodothyronine sulfotransferases and uridine 5'-diphospho-glucuronyltransferase, as well as our newly developed radiometric assays for iodothyronine deiodinases of types 1, 2 and 3, using 125I-labelled iodothyronines of high specific radioactivity as substrates.
Related projects:	Proteins in metabolism and interaction of organisms with the environment