Comparison of gene expression profiling between amplified clinical samples using cDNA microarrays

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Authors

KRONTORÁD KOUTNÁ Irena POTĚŠILOVÁ Michaela PETERKOVÁ Martina TESAŘOVÁ Lenka RUČKA Zdeněk

Year of publication 2010
Type Article in Proceedings
Conference Advances in Molecular and Cancer Biology, 2010
MU Faculty or unit

Faculty of Informatics

Citation
Field Genetics and molecular biology
Keywords microarrays - cDNA - amplification - pulmonary cells
Description Comparison of gene expression profiles obtained using microarrays technology applied to clinical samples ranks among current issues especially if amplification is needed for obtaining a sufficient amount of input RNA. We have designed a protocol for (i) convenient isolation of pure RNA from a small amount of cells, (ii) precise RNA amplification and (iii) determination of obtained microarray data. Pulmonary cells represented the clinical samples of given characteristic in our study.
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