Biomarkers of lymph node metastasis in low-grade breast cancer: An integrated, proteomics-based approach
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| Year of publication | 2010 |
| MU Faculty or unit | |
| Citation | |
| Description | Our effort has been focused on biomarker discovery in the set of 96 breast cancer tumors divided into groups according to grade and presence or absence of lymph node metastases. Three proteomics approaches were involved in complex protein analysis of tissue lysates: (i) SELDI-TOF MS enabled us to quantify 130 intact protein peaks. One protein peak correlating with lymph node metastases was detected and identified. (ii) Selected set of tumors was analyzed using iTRAQ-2DLC-MS/MS approach. This approach provides a simultaneous identification and quantitation of more than 600 proteins. (iii) Set of selected tumor lysates was analyzed also using 2-D SDS-PAGE. The expression variability of 26 selected gene products was validated using qRT-PCR. Generally, our results indicate the differential expression of (i) cytoskeletal proteins, (ii) AGR protein family members and (iii) proteins involved in heme biosynthesis. |
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